GARP (LRRC32) is a transmembrane protein that binds latent-TGF-β1 and tethers it on the Treg surface.
and has been proved to promote the activation and secretion of transforming growth factor β (TGF-β).
The expression of GARP is highly on the surface activated Tregs and increases the suppressive function of Tregs.
Additionally, GARP can bind to latent transforming growth factor β (TGF-β), thus promoting secretion and activation of TGF-β.
TGF-β plays a critical rule for homeostasis and function of Tregs.
Notably, it has been also observed that fibroblasts and endothelial cell lines that express GARP/latent TGF-β1 complexes do not activate TGF-β1.
However, it cannot be excluded that specific stimuli are required to trigger TGF-β1 activation from complexes on the surface of these cell types.
特徴
Cynomolgus LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.1 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature TGF-beta 1) respectively.
LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag.
The reducing (R) Heterotrimer protein migrates as 70 kDa (LRRC32), 37-43 kDa (LAP) and 13 kDa (mature TGF-beta 1) due to glycosylation respectively.
仕様
容量：1mg
Source：Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free (GA1-C52W7) is expressed from human 293 cells (HEK293). It contains AA Ala 18 -Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1) (Accession # A0A2K5X2X9-1 (LRRC32) & A0A2K5TJB2|Release 2019_11(TGF-beta 1)).
Species Reactivity：Cynomolgus
Host：HEK293
Tag：C-10×His | Native
Exp Region：Ala 18 -Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1)
