Matrix metalloproteinase-2 (MMP-2) is also known as 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A and CLG4A, which belongs to the peptidase M10A family.
MMP-2 cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.
MMP2 involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture.
As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction.
Also cleaves KISS at a Gly-|-Leu bond.
Appears to have a role in myocardial cell death pathways.
Contributes to myocardial oxidative stress by regulating the activity of GSK3beta.
Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin.
特徴
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 50.0 kDa.
The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：500ug
Source：Mouse MMP-2 (30-460), His Tag (MM2-M52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 30 - Thr 460 (Accession # P33434-1). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.
Species Reactivity：Mouse
Host：HEK293
Tag：C-10×His
Exp Region：Ala 30 - Thr 460
Format：Liquid
Conjugate：Unconjugated
Swiss-Prot：P33434-1
Protein ID：NP_032636.1
Endotoxin：1.0 EU per μg
Purity：85%
Buffer：50 mM Tris, 150 mM NaCl, 20% glycerol, pH7.5
