Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis.
SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate.
SPAM-1 / PH20 involved in sperm-egg adhesion.
Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida.
The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation.
SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
特徴
This protein carries no "tag".
The protein has a calculated MW of 52.6 kDa.
The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：200ug
Source：Human PH20, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).
