Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development.
HGFR protein possesses tyrosine-kinase activity.
The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor.
HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin.
Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth.
Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis).
HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain.
Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body.
Various mutations in the HGFR gene are associated with papillary renal carcinoma.
HGFR mediates a complex program known as invasive growth.
Activation of HGFR triggers mitogenesis, and morphogenesis.
特徴
Mouse HGF R, Fc Tag is fused with Fc fragment of human IgG1 at the C-terminus.
The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain.
Each α and β chain has a calculated MW of 32.1 kDa (α chain) and 95.1 kDa (β chain Fc chimera).
The protein migrates as 40-45 kDa (α chain) and 125-135 kDa (β chain Fc chimera) under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：100ug
Source：Mouse HGF R, Fc Tag (MET-M5254) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Ala 931 (Accession # P16056-1).
Species Reactivity：Mouse
Host：HEK293
Tag：C-Fc
Exp Region：Glu 25 - Ala 931
Format：Powder
Conjugate：Unconjugated
Swiss-Prot：P16056-1
Endotoxin：1.0 EU per μg
Purity：95%
Buffer：50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
