Osteopontin (OPN) is also known as Secreted phosphoprotein 1 (SPP1), Bone sialoprotein 1, Nephropontin, Urinary stone protein, Uropontin, BNSP, which belongs to the osteopontin family.
OPN / SPP1 is a highly negatively charged, extracellular matrix protein that lacks an extensive secondary structure.
Full length OPN (OPN-FL) can be modified by thrombin cleavage, which exposes a cryptic sequence, SVVYGLR on the cleaved form of the protein known as OPN-R.
Osteopontin / SPP-1 is biosynthesized by a variety of tissue types.
OPN is the ligand for integrin alpha-V/beta-3.
OPN / SPP1 binds tightly to hydroxyapatite and appears to form an integral part of the mineralized matrix.
OPN / SPP1 probably important to cell-matrix interaction.
OPN / SPP1 acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
特徴
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).
The protein has a calculated MW of 35.7 kDa.
The protein migrates as 16 kDa, 23 kDa, 28 kDa and 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic processing of several intracellular enzymes.
仕様
容量：200ug
Source：Biotinylated Human Osteopontin, His,Avitag (OPN-H82E8) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # P10451-5).
