Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites.
The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.
In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases).
VHL is involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
It seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions.
Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases.
特徴
Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag, produced by co-expression of Elongin B and Elongin C and VHL, has a calculated MW of 14.5 kDa (Elongin B) and 13.8 kDa (Elongin C) and 25.8 kDa (VHL).
Subunit Elongin B is fused with a Strep II tag at the N-terminus and Elongin C is fused with a Strep II tag at the N-terminus and VHL is fused with a polyhistidine tag at the N-terminus.
The protein migrates as 17 kDa (Elongin B) and 14 kDa (Elongin C) and 30 kDa (VHL) under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：50ug
Source：Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag (ELL-H5595) is expressed from Baculovirus-Insect cells. It contains AA Asp 2 - Gln 118 (Elongin B) & Asp 2 - Cys 112 (Elongin C) & Pro 2 - 213 Asp (VHL) (Accession # Q15370-1 (Elongin B) & Q15369-1 (Elongin C) & P40337-2 (VHL)).
Species Reactivity：Human
Host：InsectCells
Tag：N-Strep II | N-Strep II | N-10×His
Exp Region：Asp 2 - Gln 118 (Elongin B) & Asp 2 - Cys 112 (Elongin C) & Pro 2 - 213 Asp (VHL)
Format：Liquid
Conjugate：Unconjugated
Swiss-Prot：Q15370-1 (Elongin B) & Q15369-1 (Elongin C) & P40337-2 (VHL)
Protein ID：NP_009039.1 | NP_005639.1 | NP_000542.1
Endotoxin：1.0 EU per μg
Purity：95%
Buffer：50 mM HEPES, 200 mM NaCl, 20% Glycerol, 1 mM TCEP, pH7.5
