83-3855-45　Biotinylated ENPP3 protein, His,Avitag （active enzyme） 25ug　EN3-H82E5-25ug

特徴

• 背景
• The human NPP family contains seven members which can be classified into two groups according to their substrate preferences.
• The first class comprises the nucleotide-degrading proteins NPP1, 3 and 4.
• NPP3 (CD203c, ENPP3) is expressed in multiple organs, including on epithelial and mucosal surfaces, and notably on basophils and mast cells.
• Activation of basophils by antigen-bound IgE leads to release of inflammatory mediators and rapid upregulation of NPP3 to the cell surface.
• This protein is in fact a common marker for diagnosing allergen sensitivity with patient basophils by flow cytometry.
• Basophils and mast cells mediate the response to certain pathogens, as well as acute and chronic allergic reactions.
• Following activation, these cells release ATP, which further stimulates them in an autocrine manner.
• NPP3 upregulation serves to degrade ATP and suppress chronic allergic inflammation (but not the acute response).
• 特徴
• This protein carries an Avi tag (Avitag) at the N-terminus, followed by a polyhistidine tag.
• The protein has a calculated MW of 98.6 kDa.
• The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.