Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin.
uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).
特徴
This protein carries a polyhistidine tag at the C-terminus.
The active form of Human PLAU is a disulfide-linked heterodimer composed of long chain A (Ser 21 - Phe 177) and chain B (Ile 179 - Leu 431) with calculated MW of 17.8 kDa and 29.2 kDa.
The long chain A is further cleaved to yield a short chain A (Lys 156 - Phe 177) and N-terminal fragment (Ser 21 - Lys 155) with calculated MW of 15.3 kDa.
The protein migrates as 17 kDa (N-terminal fragment), 32-35 kDa (chain B) and 45-50 kDa (long chain A & chain B) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
仕様
容量：50ug
Source：Human PLAU, His Tag (activated by trypsin) (PLU-H5228) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # P00749-1).
