Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis.
SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate.
SPAM-1 / PH20 involved in sperm-egg adhesion.
Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida.
The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation.
SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
特徴
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 53.0 kDa.
The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：1mg
Source：Human PH20, His Tag (PH0-H5225) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Tyr 482 (Accession # P38567-1).
