Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily.
Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum.
This protein plays a major regulatory role in cholesterol homeostasis.
PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation.
PCSK9 may also have a role in the differentiation of cortical neurons.
Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
特徴
This protein carries a polyhistidine tag at the C-terminus.
This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain.
The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.2 kDa respectively.
The protein migrates as 17 kDa and 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The D374Y mutation results in higher affinity of PCSK9 for LDLR.
仕様
容量：50ug
Source：Human PCSK9 (D374Y), His Tag (PCY-H5225) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 (D374Y)).
