The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13.
and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2.
It belongs to the large class of tyrosine kinase receptors.
This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin.
IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor.
The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation.
IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation.
There is a 60% homology between IGF1R and the insulin receptor.
In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains.
This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
特徴
This protein carries a polyhistidine tag at the C-terminus.
This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.7 kDa and 24.3 kDa respectively.
The protein migrates as 45-55 kDa (partial β chain), 100-125 kDa (α chain) and 150 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：100ug
Source：Mouse IGF-I R, His Tag (IGR-M5223) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1).
