Protease Recombinant is fusion protein of GST and human rhinovirus (HRV) type 14 3C protease.Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃, even retaining robust activity at 4℃.
Thus, cleavage can be performed at low temperature to enhance the stability of the target protein.
The catalytic activity is insensitive to organic solvents (up to 10%); however, it can be strongly stimulated by high concentration of anions such as sulfate.
特徴
This protein carries a GST tag at the N-terminus.
The protein has a calculated MW of 46.0 kDa.
The protein migrates as 44-46 kDa under reducing (R) condition (SDS-PAGE).
仕様
容量：5000U
Source：HRV-3C Protease Cleavage Enzyme, GST Tag is a recombinant form of human rhinovirus (HRV) type 14 3C protease produced in Escherichia coli cells at ACRObiosystems.
Species Reactivity：Human rhinovirus
Host：E. coli
Tag：N-GST
Exp Region：Gly 1 - Gln 182
Format：Powder
Conjugate：Unconjugated
Swiss-Prot：P35613-2
Protein ID：NP_940991.1
Endotoxin：1.0 EU per μg
Purity：95%
Buffer：50 mM Tris,150 mM NaCl,1 mM EDTA,0.05% Tween20,1 mM DTT,PH8.0
