Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.
Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ.
CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation).
CD86 works in tandem with CD80 to prime T cells.
Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival.
Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages.
CD86 thus is regarded as a promising candidate for immune therapy.
CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
特徴
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.5 kDa.
The protein migrates as 66-100 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
仕様
容量：1mg
Source：Human B7-2, Fc Tag, premium grade (CD6-H5257) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Pro 247 (Accession # AAH40261).
Species Reactivity：Human
Host：HEK293
Tag：C-Fc
Exp Region：Leu 26 - Pro 247
Format：Powder
Conjugate：Unconjugated
Swiss-Prot：P42081
Protein ID：AAH40261
Endotoxin：0.01 EU per μg
Purity：95%
Buffer：50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5
