Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process.
C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond.
C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.
C5b has a transient binding site for C6.
The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor.
C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
特徴
This protein carries a polyhistidine tag at the C-terminus.The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain.
Each α and β chain has a calculated MW of 74.1 kDa (β chain) and 114.8 kDa (α chain).
The protein migrates as 70 kDa (β chain) and 110 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：1mg
Source：Rat Complement C5, His Tag (CO5-R52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 1688 (Accession # XP_001079130.2).
