T-Pro Ponceau S Stain Solution is a ready‑to‑use membrane stain for evaluating protein transfer efficiency or for use in total protein normalization for western blot analysis.
The stain has minimal nonspecific interactions with nitrocellulose or PVDF membranes, providing a reliable method for visualization of membrane‑bound proteins.
Staining with Ponceau S Stain Solution results in pink-reddish protein bands that are easily photographed and reversed in less than 5 minutes.
Subsequent immunodetection is unaffected, as the stain does not alter membrane‑bound proteins.