T-Pro BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid （BCA） for the colorimetric detection and quantitation of total protein.
This method was base on the reduction of Cu+2 to Cu+1 by protein in an alkaline medium with the highly sensitive and selective colorimetric detection.
The purple-colored reaction product of this assay is formed and exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range （20-2,000 μg/ml）.
The reaction occurred in T-Pro BCA Protein Assay Kit is not a true end-point method; that is, the final color continues to develop.
However, following incubation, the rate of continued color development is sufficiently slow to allow large numbers of samples to be assayed together.
Thus, protein concentrations generally are determined and reported with reference to standards of bovine serum albumin （BSA）.
A series of dilutions of known concentration are prepared from the protein and assayed alongside the unknown（s） before the concentration of each unknown is determined based on the standard curve.
