83-2272-22 KRIBIOLISA CHO Host Cell DNA Kit 100 reactions KBBR02

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  • Description:Immunoassay for the measurement of HEK Host Cell Proteins.
  • For the production of biotechnological products, expression of therapeutic proteins in CHO cells is
  • commonly used.
  • Due to which there is a possibility of DNA contamination from the host cells in the
  • products which are manufactured.
  • However, the limit of DNA contamination from the cell lines has
  • been set by regulatory agencies According to WHO the limit of DNA contamination should not
  • exceed 10ng/dose in the products manufactured.
  • One of the most common method which is
  • widely used for detection of host cell DNA contamination by biopharmaceutical manufacturers is
  • the PicoGreenTM Dye based assay.
  • The research and development department at Krishgen
  • Biosystems has designed a kit to detect and quantify host cell DNA in the products manufactured
  • by recombinant expression in CHO cell lines based on PicoGreenTM Dye assay.
  • About the kit:
  • - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
  • - Recovery rates are between 85 - 115%
  • - Ready to use with a standard protocol with break-apart pre-coated wells
  • - Validated as per US FDA guidelines for Bioassays
  • - Optimized for matrix effects to ensure higher sensitivity.
  • - Shelf life: 1 year
  • The Krishgen CHO Host Cell DNA Kit is based on the DNA dye binding assay which utilizes
  • PicoGreenTM dye.
  • The DNA samples and the standards are reacted with PicoGreenTM dye, which is a
  • DNA interculator that binds strongly to the double stranded DNA.
  • Upon binding to DNA, PicoGreenTM
  • dye fluoresces with an excitation of 485 nm and emission of 525 nm.
  • The intensity of the fluorescent
  • signal is proportional to the quantity of DNA in the standard or the samples.


  • サイズ:100 reactions
  • Reactivity:Human
  • アプリケーション:Assay
  • Sensitivity:Please refer to the current IFU for the updated calibrator range and sensitivity.
  • Calibration Range:3.13ng/ml - 200 ng/ml
  • サンプルタイプ:Cell Culture Supernatant and Other Biological Preparations
  • データシート:https://krishgen.com/upload/datasheets/KBBR02-CHO-HCD.pdf
  • 測定方法:Colorimetric, 450nm
  • この商品は法規制を確認しておりません。(法規制によって販売できない場合もございます)

アズワン品番 83-2272-22
型番 KBBR02
入り数 1個
標準価格 108,000円(税抜)
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