83-2272-22　［受注停止］KRIBIOLISA CHO Host Cell DNA Kit 100 reactions　KBBR02

特徴

• Description：Immunoassay for the measurement of HEK Host Cell Proteins.
• For the production of biotechnological products, expression of therapeutic proteins in CHO cells is
• commonly used.
• Due to which there is a possibility of DNA contamination from the host cells in the
• products which are manufactured.
• However, the limit of DNA contamination from the cell lines has
• been set by regulatory agencies According to WHO the limit of DNA contamination should not
• exceed 10ng/dose in the products manufactured.
• One of the most common method which is
• widely used for detection of host cell DNA contamination by biopharmaceutical manufacturers is
• the PicoGreenTM Dye based assay.
• The research and development department at Krishgen
• Biosystems has designed a kit to detect and quantify host cell DNA in the products manufactured
• by recombinant expression in CHO cell lines based on PicoGreenTM Dye assay.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Krishgen CHO Host Cell DNA Kit is based on the DNA dye binding assay which utilizes
• PicoGreenTM dye.
• The DNA samples and the standards are reacted with PicoGreenTM dye, which is a
• DNA interculator that binds strongly to the double stranded DNA.
• Upon binding to DNA, PicoGreenTM
• dye fluoresces with an excitation of 485 nm and emission of 525 nm.
• The intensity of the fluorescent
• signal is proportional to the quantity of DNA in the standard or the samples.