83-2272-21　［受注停止］KRIBIOLISA E.coli Host Cell DNA Kit 100 reactions　KBBR01

特徴

• Description：For the production of biotechnological products, expression of therapeutic proteins in E.coli cells is commonly used.
• Due
• to which there is a possibility of DNA contamination from the host cells in the products which are manufactured.
• However, the limit of DNA contamination from the cell lines has been set by regulatory agencies According to WHO the
• limit of DNA contamination should not exceed 10 ng/dose in the products manufactured.
• One of the most common
• method which is widely used for detection of host cell DNA contamination by biopharmaceutical manufacturers is the
• PicoGreenTM Dye based assay.
• The research and development department at Krishgen Biosystems has designed a kit
• to detect and quantify host cell DNA in the products manufactured by recombinant expression in E.coli cell lines based
• on PicoGreenTM Dye assay.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Krishgen E.coli Host Cell DNA Kit is based on the DNA dye binding assay which utilizes PicoGreenTM dye.
• The
• DNA samples and the standards are reacted with PicoGreenTM dye, which is a DNA intercalator that binds strongly to
• the double stranded DNA.
• Upon binding to DNA, PicoGreenTM dye fluoresces with an excitation of 485 nm and emission
• of 525 nm.
• The intensity of the fluorescent signal is proportional to the quantity of DNA in the standard or the samples.