83-2272-20　［受注停止］KRIBIOLISA HEK HCP ELISA 1 x 96 wells　KBBP15

特徴

• Description：Immunoassay for the measurement of HEK Host Cell Proteins.
• The KRIBIOLISA ELISA kits are used for assessing the specific biomarker in samples analytes.
• The
• kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity
• compared to conventional competitive ELISA kits which employ only one antibody.
• Double antibodies
• are used in this kit.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• This assay is based on the Sandwich ELISA procedure.
• Samples containing HEK HCPs are reacted
• simultaneously with a horseradish peroxidase （HRP） enzyme labeled anti-HEK antibody in microtiter
• strips coated with an affinity purified capture anti-HEK antibody.
• The immunological reactions result in
• the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody.
• The
• microtiter strips are washed to remove any unbound reactants.
• The substrate, tetramethylbenzidine
• （TMB） is then reacted.
• The amount of hydrolyzed substrate is read on a microtiter plate reader and is
• directly proportional to the concentration of HEK HCPs present.