83-2272-19 KRIBIOLISA Protein A ELISA 1 x 96 wells KBBP11

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  • Description:Immunoassay for the measurement Natural & Structurally conserved
  • Recombinant Protein A in samples containing Human immunoglobulin
  • A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals.
  • These antibodies are
  • generally purified through protein A column chromatography.
  • This method of purification has the potential with
  • contamination of protein A, which might get leached out of the column and co-eluted with purified antibody.
  • This
  • may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody.
  • The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as
  • well as screening product prior to its use.
  • About the kit:
  • - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
  • - Recovery rates are between 85 - 115%
  • - Ready to use with a standard protocol with break-apart pre-coated wells
  • - Validated as per US FDA guidelines for Bioassays
  • - Optimized for matrix effects to ensure higher sensitivity.
  • - Shelf life: 1 year
  • This assay is based on the principle of Sandwich ELISA.
  • Samples containing Protein A are reacted with anti-
  • Protein A antibody coated on the microtitre plates.
  • The wells are washed to remove any unbound reactants as per
  • the wash procedure.
  • Anti-Protein A HRP antibody is added to the wells and incubated.
  • .
  • The wells are washed to
  • remove any unbound reactants as per the wash procedure.
  • simultaneously in the microtiter wells already coated
  • with affinity purified capture anti-Protein A antibody.
  • The substrate 3,3',5,5' Tetramethyl Benzidine is then reacted.
  • The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the
  • concentration of Protein A present.


  • サイズ:1 x 96 wells
  • Reactivity:Human
  • アプリケーション:Assay
  • Sensitivity:Please refer to the current IFU for the updated calibrator range and sensitivity.
  • Calibration Range:0 pg/ml - 4000 pg/ml
  • サンプルタイプ:Cell Culture Supernatant and Other Biological Preparations
  • データシート:https://krishgen.com/upload/datasheets/Protein_A_ELISA_KBBP11ver2_0.pdf
  • 測定方法:Colorimetric, 450nm
  • この商品は法規制を確認しておりません。(法規制によって販売できない場合もございます)

アズワン品番 83-2272-19
型番 KBBP11
入り数 1個
標準価格 94,000円(税抜)
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