83-2272-19　［受注停止］KRIBIOLISA Protein A ELISA 1 x 96 wells　KBBP11

特徴

• Description：Immunoassay for the measurement Natural ＆ Structurally conserved
• Recombinant Protein A in samples containing Human immunoglobulin
• A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals.
• These antibodies are
• generally purified through protein A column chromatography.
• This method of purification has the potential with
• contamination of protein A, which might get leached out of the column and co-eluted with purified antibody.
• This
• may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody.
• The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as
• well as screening product prior to its use.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• This assay is based on the principle of Sandwich ELISA.
• Samples containing Protein A are reacted with anti-
• Protein A antibody coated on the microtitre plates.
• The wells are washed to remove any unbound reactants as per
• the wash procedure.
• Anti-Protein A HRP antibody is added to the wells and incubated.
• .
• The wells are washed to
• remove any unbound reactants as per the wash procedure.
• simultaneously in the microtiter wells already coated
• with affinity purified capture anti-Protein A antibody.
• The substrate 3,3',5,5' Tetramethyl Benzidine is then reacted.
• The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the
• concentration of Protein A present.