83-2272-18　［受注停止］KRIBIOLISA Bovine Serum Albumin ELISA * 1 x 96 wells　KBBP10

特徴

• Description：Enzyme Immunoassay for the Quantitative Determination of Bovine Serum
• Albumin in serum, plasma and other biological samples
• The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be
• serum, plasma and cell culture supernatant as validated with the kit.
• The kit employs a sandwich ELISA technique
• which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits
• which employ only one antibody.
• Double antibodies are used in this kit.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The method employs sandwich ELISA technique.
• Monoclonal antibodies are pre-coated onto microwells.
• Samples
• and standards are pipetted into microwells and Bovine Serum Albumin present in the sample are bound by
• the antibodies.
• Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to
• form a complex.
• After washing microwells in order to remove any non-specific binding, the substrate solution
• （TMB） is added to microwells and color develops proportionally to the amount of Bovine Serum Albumin in the
• sample.
• Color development is then stopped by addition of stop solution.
• Absorbance is measured at 450 nm.