83-2272-17　［受注停止］KRIBIOLISA Human IgA ELISA 1 x 96 wells　KBBP09

特徴

• Description：Immunoassay for quantitative determination of Human IgA in cell culture
• supernatant and other biological preparations
• Human IgA is an antibody playing a critical role in mucosal immunity, and found in the form of secretory IgA
• polymer in the secretions.
• Presence of such a trace contaminants in the products may lead to potential health
• hazards, especially when the product is intended for therapeutic use.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• This assay is based on the Sandwich ELISA procedure.
• Samples containing IgA react with already coated
• affinity purified capture anti-Human IgA antibody and bind to them.
• Plates are washed with wash buffer to
• remove unbound reactants.
• Biotinylated Anti-Human IgA is added leading to formation of a sandwich complex
• of solid phase antibody-Human IgA-enzyme labeled antibody.
• The wells are washed to remove any unbound
• reactants as per the wash procedure.
• Streptavidin：HRP conjugate is added which binds to Biotinylated Anti-
• Human IgA complex.
• The wells are washed to remove any unbound reactants as per the wash procedure.
• The
• substrate 3, 3',5, 5' Tetra Methyl Benzidine is then reacted.
• The amount of hydrolyzed substrate is read on a
• microtiter plate reader and it is directly proportional to the concentration of Human IgA present in the samples.