83-2272-16 [受注停止]KRIBIOLISA Human Serum Albumin ELISA 1 x 96 wells KBBP07
[KRISHGEN BioSystems]
特徴
- Description:Immunoassay for the measurement of Human Serum Albumin (HSA)
- A variety of products, manufactured using the biotechnology route such as cell or tissue culture may result in
- residual contamination of the end-product.
- Trace contamination of HSA in pharmaceutical and other biological
- products is one such area.
- Such trace contaminants may lead to potential health hazards in humans, especially
- when the product is intended for therapeutic use.
- About the kit:
- - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
- - Recovery rates are between 85 - 115%
- - Ready to use with a standard protocol with break-apart pre-coated wells
- - Validated as per US FDA guidelines for Bioassays
- - Optimized for matrix effects to ensure higher sensitivity.
- - Shelf life: 1 year
- This assay is based on the Sandwich ELISA method.
- Samples containing Human Albumin are reacted with
- microtiter wells pre-coated with affinity purified capture HSA antibody followed by washing and then detected
- using detection antibody and HRP Conjugated antibody.
- This immunological reaction results in formation of an
- immuno complex of solid phase.
- The wells are washed to remove any unbound reactants.
- The substrate 3, 3', 5,
- 5' Tetramethyl Benzidine is then reacted.
- The amount of hydrolyzed substrate is read on a microtiter plate reader
- and it is directly proportional to the concentration of HSA present.
仕様
- サイズ:1 x 96 wells
- Reactivity:Human
- アプリケーション:Assay
- Sensitivity:Please refer to the current IFU for the updated calibrator range and sensitivity.
- Calibration Range:0.5 ng/ml - 100 ng/ml
- サンプルタイプ:Cell Culture Supernatant and Other Biological Preparations
- データシート:https://krishgen.com/upload/datasheets/KBBP07-HSA-ELISA-kitinsert-Ver4.0.pdf
- 測定方法:Colorimetric, 450nm
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