83-2272-15 [受注停止]KRIBIOLISA Ovalbumin ELISA 1 x 96 wells KBBP06
[KRISHGEN BioSystems]
特徴
- Description:Immunoassay for the quantification of Ovalbumin
- Egg allergies occur in a small size of the population.
- As some of the vaccines like influenza and yellow fever
- vaccines are made in eggs, egg proteins (primarily Ovalbumin) are present in the final product.
- Residual quantities
- of egg proteins found in the vaccines may induce severe and rarely fatal hypersensitivity reactions in children with
- egg allergies.
- The KRIBIOLISA Ovalbumin ELISA enables estimation of Ovalbumin found as impurities in vaccines
- and cell culture supernatants.
- About the kit:
- - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
- - Recovery rates are between 85 - 115%
- - Ready to use with a standard protocol with break-apart pre-coated wells
- - Validated as per US FDA guidelines for Bioassays
- - Optimized for matrix effects to ensure higher sensitivity.
- - Shelf life: 1 year
- This assay is based on the Sandwich ELISA method.
- Samples containing Ovalbumin are reacted with microtiter
- wells pre-coated with affinity purified capture Ovalbumin antibody followed by washing and then detected using
- detection antibody and HRP Conjugated antibody.
- This immunological reaction results in formation of an immuno
- complex of solid phase.
- The wells are washed to remove any unbound reactants.
- The substrate 3, 3', 5, 5'
- Tetramethyl Benzidine is then reacted.
- The amount of hydrolyzed substrate is read on a microtiter plate reader
- and it is directly proportional to the concentration of Ovalbumin present.
仕様
- サイズ:1 x 96 wells
- Reactivity:Human
- アプリケーション:Assay
- Sensitivity:Please refer to the current IFU for the updated calibrator range and sensitivity.
- Calibration Range:0.5 ng/ml - 100 ng/ml
- サンプルタイプ:Cell Culture Supernatant and Other Biological Preparations
- データシート:https://krishgen.com/upload/datasheets/KBBP06-Ovalbumin-ELISA-kitinsert-Ver2.0.pdf
- 測定方法:Colorimetric, 450nm
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