83-2272-13 KRIBIOLISA CHO HCP ELISA 1 x 96 wells KBBP03

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  • Description:Immunoassay for the measurement of CHO Host Cell Proteins in
  • biological preparations and cell culture supernatant
  • A variety of proteins which are used as therapeutic agents in humans and animals are produced through
  • recombinant expression in Chinese Hamster Ovary (CHO) cells.
  • The manufacturing and purification process of
  • these products tends to leave the potential for contamination by Host Cell Proteins (HCPs) from CHO cells,
  • which may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the therapeutic
  • agent.
  • The simple, objective and semi-quantitative ELISA is a highly sensitive method that aids in purification
  • process development, process control, quality control and product release testing optimally.
  • About the kit:
  • - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
  • - Recovery rates are between 85 - 115%
  • - Ready to use with a standard protocol with break-apart pre-coated wells
  • - Validated as per US FDA guidelines for Bioassays
  • - Optimized for matrix effects to ensure higher sensitivity.
  • - Shelf life: 1 year
  • This assay is based on the Sandwich ELISA procedure.
  • Samples containing CHO HCPs are reacted with
  • already coated affinity purified capture anti-CHO HCP antibody.
  • Following an incubation period unbound
  • components are removed by a washing step.
  • Then the anti-CHO HCP: HRP is added.
  • This immunological
  • reaction results in formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody.
  • The
  • wells are again washed to remove any unbound reactants.
  • The TMB substrate is then added to the well.
  • The
  • amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the
  • concentration of CHO HCPs present.¥


  • サイズ:1 x 96 wells
  • Reactivity:Human
  • アプリケーション:Assay
  • Sensitivity:5 ng/ml
  • Calibration Range:6.25 ng/ml - 400 ng/ml
  • サンプルタイプ:Cell Culture Supernatant and Other Biological Preparations
  • データシート:https://krishgen.com/upload/datasheets/KBBP03-CHO-HCP-ELISA-IFU.pdf
  • 測定方法:Colorimetric, 450nm
  • この商品は法規制を確認しておりません。(法規制によって販売できない場合もございます)

アズワン品番 83-2272-13
型番 KBBP03
入り数 1個
標準価格 116,000円(税抜)
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