83-2272-07　［取扱停止］KRIBIOLISA Bovine IgG ELISA 1 x 96 wells　KBI1006

特徴

• Description：Immunoassay for the quantification of Bovine IgG
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The method employs the quantitative sandwich enzyme immunoassay technique.
• A polyclonal antibody specific
• to Bovine IgG is pre-coated onto microwells.
• Samples and standards are pipetted into microwells and Bovine
• IgG present in the sample are bound by the capture antibody.
• Then, a HRP （horseradish peroxidase）
• conjugated anti-Bovine IgG （H+L） antibody is pipetted and incubated simultaneously with samples.
• After
• washing microwells in order to remove any non-specific binding, the ready to use substrate solution （TMB） is
• added to microwells and color develops proportionally to the amount of Bovine IgG in the sample.
• Color
• development is then stopped by addition of stop solution.
• Absorbance is measured at 450 nm.