特徴

• Description：Enzyme Immunoassay for the Quantitative Determination of
• Kanamycin in cell culture supernatant and other purified systems
• including vaccines
• Kanamycin or kanamycin A is an aminoglycoside bacteriocidal antibiotic, used to treat a wide variety of
• infections and tuberculosis.
• Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its
• most commonly used form is kanamycin sulfate.
• Kanamycin is commonly used as an antibiotic during the cell culture process.
• Regulatory authorities across the
• world have restricted and sought to quantify the Kanamycin residue in view of its potent action on human
• beings in the in-process and finished pharmaceutical products.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• KRIBIOLISA Kanamycin ELISA kit is based on indirect-competitive ELISA.
• The microtiter wells are coated
• with antigen.
• Kanamycin residue in the sample competes with antigen coated on the microtitre plate for the
• antibody.
• After the addition of biotinylated antibody and enzyme conjugate, an immune complex will form.
• After
• washing, addition of TMB Substrate is used to show the color.
• Absorbance of the sample is inversely
• proportional to the Kanamycin residue in the samples.
• Color development is stopped by addition of stop
• solution.
• Absorbance is measured at 450 nm