83-2271-76　［受注停止］FastNuclease Endonuclease Serratia Marcescens ELISA 1 x 96 wells　KBBA36

特徴

• Description：ELISA for Quantitative Determination of Serratia endonuclease in Cell
• Culture and Biological Samples
• Serratia marcescens nuclease originates from Gram negative bacteria S.
• marcescens and heads a family of
• homological non-specific nucleases that are widely spread in the world.
• Serratia nuclease is most studied one
• and it is capable to cleavage both RNA and DNA in either single or double stranded form.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Endonuclease Serratia marcescens ELISA is a competitive immunoassay for the determination of Serratia
• endonuclease in sample.
• The secondary antibodies specific to anti-endonuclease are coated on to the microtiter
• plate.
• A constant concentration of HRP labeled endonuclease and varying concentration of standard or sample
• containing endonuclease compete for binding to anti-endonuclease antibodies.
• This immune complex is captured
• by the coated secondary antibody.
• After incubation and washing, the unbound labeled enzyme is removed.
• Then
• addition of substrate develops blue color during incubation period and the reaction is stopped after the addition of
• stop solution with development of yellow color.
• The intensity of the color generated is inversely proportional to the
• amount of endonuclease in the sample.