特徴

• Description：Enzyme Immunoassay for the quantitative determination of PEG in
• serum, plasma and cell culture supernatant
• PEG is generally considered biologically inert and safe.
• However, studies of clinical safety are generally based
• on adults, not children.
• The FDA has been asked to investigate the possible effects of PEG in laxatives for
• children.
• Also, a minority of people are allergic to it.
• Allergy to PEG is usually discovered after a person has
• been diagnosed with an allergy to an increasing number of seemingly unrelated products, including processed
• foods, cosmetics, drugs, and other substances that contain PEG or were manufactured with PEG.
• When PEG
• is chemically attached to therapeutic molecules （such as protein drugs or nanoparticles）, it can be antigenic
• sometimes, stimulating an anti-PEG antibody response in some patients.
• This effect has only been shown for a
• few of the many available PEGylated therapeutics, but it has significant effects on clinical outcomes of affected
• patients.
• Other than these few instances where patients have anti-PEG immune responses, it is generally
• considered to be a safe component of drug formulations.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The method employs the quantitative sandwich enzyme immunoassay technique.
• Anti- PEG antibody is
• pre-coated onto microwells.
• In the first step, samples and standards are pipetted into microwells.
• If
• PEG i s present in samples or standards, it will form complex with Anti- PEG antibody, which is pre-
• coated onto microwells.
• In the second step, Anti- PEG Biotin conjugate is pipetted and incubated.
• In the
• third step, HRP Conjugate is pipetted and incubated.
• Free HRP conjugate will be removed by a washing
• step.
• In the f o u r t h s t e p , T M B substrate is added to microwells and color develops proportionally to
• the amount of PEG present in samples or standards.
• Color development is then stopped by addition of
• stop solution.
• Absorbance is measured at 450 nm.