83-2271-70　［受注停止］KRIBIOLISA FSH （Follicle Stimulating Hormone） Bioassay ELISA 1 x 96 wells　KBBA08

特徴

• Description：Immunoassay for the quantification of hFSH in human Serum
• Human follicle stimulating hormone （FSH） is a glycoprotein hormone produced by the anterior pituitary gland.
• There are three other glycoprotein hormones, namely Thyroid Stimulating Hormone, Luteinizing Hormone （both
• produced by anterior pituitary gland） and Human Chorionic Gonadotropin （produced by the placenta） which are
• structurally similar.
• Each hormone has an alpha and beta subunit.
• The α subunits of each hormone are similar
• while the β subunit is specific to each hormone.
• The α subunits contain 92 amino acids while the β subunits vary
• with each hormone.
• The β subunit of both FSH and LH contain 115 amino acids, TSH 110 amino acids, and hCG
• 147 amino acids.
• The FSH and LH hormones function differently in females and males.
• It is to be noted that in
• women the growth and maturation of the ovarian follicle is dependent on FSH, while in men both LH and FSH act
• on the testes.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The method employs the quantitative sandwich enzyme immunoassay technique.
• Antibodies to FSH are pre-
• coated onto microwells.
• Samples and standards are pipetted into microwells and anti-FSH antibodies linked to
• HRP is added to the wells.
• FSH present in the sample and standards will bind to the plate and HRP Conjugate
• simultaneously.
• Washing is done to remove the unbound samples, standards and HRP conjugate.
• The ready to
• use substrate solution （TMB） is added to microwells and color develops directly proportional to the amount of
• FSH in the standards and sample.
• Color development is then stopped by addition of stop solution.
• Absorbance is
• measured at 450 nm.