83-2271-69　［受注停止］KRIBIOLISA LH （Lutenizing Hormone） Bioassay ELISA 1 x 96 wells　KBBA07

特徴

• Description：Immunoassay for the Quantification of （hLH） in human Serum
• Luteinizing hormone （LH） is produced in both men and women from the anterior pituitary gland in response to
• luteinizing hormone releasing hormone （LH-RH or Gn-RH）, released by the hypothalamus.
• LH, also called
• interstitial cell-stimulating hormone （ICSH） in men, is a glycoprotein with a molecular weight of approximately
• 30,000 Daltons.
• It is composed of two non-covalently associated dissimilar amino acid chains, alpha and beta.
• A
• lack of secretion by the anterior pituitary may cause lower LH levels.
• As may be expected, low levels may result in
• infertility in both males and females.
• Low levels of LH may also be due to the decreased secretion of GnRH by the
• hypothalamus.
• Low LH values may therefore indicate some dysfunction of the pituitary or hypothalamus, but the
• actual source of the problem must be confirmed by other tests.10
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The method employs the quantitative sandwich enzyme immunoassay technique.
• Antibodies to LH are pre-coated
• onto microwells.
• Samples and standards are pipetted into microwells and anti-hLH antibodies linked to HRP is
• added to the wells.
• LH present in the sample and standards will bind to the plate and HRP Conjugate
• simultaneously.
• Washing is done to remove the unbound samples, standards and HRP conjugate.
• The ready to
• use substrate solution （TMB） is added to microwells and color develops directly proportional to the amount of
• LH in the standards and sample.
• Color development is then stopped by addition of stop solution.
• Absorbance is
• measured at 450 nm.