83-2271-66　［受注停止］KRIBIOLISA EPO （Erythropoietin） ELISA 1 x 96 wells　KBBA01

特徴

• Description：Enzyme Immunoassay for the quantitative determination of EPO
• （Erythropoietin） in serum, plasma and cell culture supernatant
• Erythropoietin （EPO） is a heavily glycosylated protein with a molecular weight of about 30,000 - 34,000
• Daltons.
• EPO is a polypeptide consisting of 166 amino acids, containing one O-linked and three N-linked
• carbohydrate chains.
• The recombinant EPO is a good substitute for the native protein for use in an
• immunoassay.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The method employs sandwich ELISA technique.
• Streptavidin is pre-coated onto microwells.
• Samples and
• standards are pipetted into microwells and EPO present in the sample are bound by the biotin
• labeled antibodies to the microwell.
• Anti-EPO-HRP is pipetted and incubated to form a complex.
• After
• washing microwells in order to remove any non-specific binding, the substrate solution （TMB） is added to
• microwells and color develops proportionally to the amount of EPO in the sample.
• Color development is then
• stopped by addition of stop solution.
• Absorbance is measured at 450 nm.