83-2271-56　［受注停止］KRIBIOLISA Dasatinib （SPRYCEL） ELISA 1 x 96 wells　KOD1016

特徴

• Description：Enzyme Immunoassay for the quantitative determination of Dasatinib
• in serum, plasma and cell culture supernatant
• Dasatinib, sold under the brand name Sprycel, is a targeted therapy used to treat certain cases of chronic
• myelogenous leukemia （CML） and acute lymphoblastic leukemia （ALL）.
• Specifically it is used to treat cases
• that are Philadelphia chromosome-positive （Ph+）.
• It is taken by orally.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Dasatinib ELISA is a competitive immunoassay for the determination of Dasatinib.
• A constant
• concentration of Tracer; varying concentrations of unlabeled standard and BCR-ABL Tyrosine Kinase are
• added in sequence to the uncoated microtitre plate wells and incubated.
• After 1st incubation, Anti- （BCR-ABL
• Tyrosine Kinase） Antibody is dispensed to all wells of uncoated Microtitre plate containing the reaction
• mixture and incubated.
• After 2nd incubation, all reaction mixtures are transferred to the respective wells of
• coated microtitre plate and incubated.
• After washing, Streptavidin HRP conjugate is added and incubated.
• Captured Tracer is subsequently bound by streptavidin HRP which produces a soluble colored product after
• addition of TMB substrate.
• The enzyme reaction is stopped by dispensing of stop solution into the wells.The
• optical density （OD） of the solution at 450 nm is inversely proportional to the amount of bound Dasatinib
• molecule present in standards or samples.