83-2271-52　［受注停止］KRIBIOLISA Nilotinib （TASIGNA） ELISA 1 x 96 wells　KOD1012

特徴

• Description：Enzyme Immunoassay for the quantitative determination of Nilotinib
• in serum, plasma and cell culture supernatant
• Nilotinib （AMN107, trade name Tasigna）, in the form of the hydrochloride monohydrate salt, is a small-
• molecule tyrosine kinase inhibitor approved for the treatment of imatinib-resistant chronic myelogenous
• leukemia.
• Structurally related to imatinib, it was developed based on the structure of the Abl-imatinib complex
• to address imatinib intolerance and resistance.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Nilotinib ELISA is a competitive immunoassay for the determination of Nilotinib.
• A constant
• concentration of Tracer; varying concentrations of unlabeled standard and BCR-ABL Tyrosine Kinase are
• added in sequence to the uncoated microtitre plate wells and incubated.
• After 1st incubation, Anti- （BCR-ABL
• Tyrosine Kinase） Antibody is dispensed to all wells of uncoated Microtitre plate containing the reaction
• mixture and incubated.
• After 2nd incubation, all reaction mixtures are transferred to the respective wells of
• coated microtitre plate and incubated.
• After washing, Streptavidin HRP conjugate is added and incubated.
• Captured Tracer is subsequently bound by streptavidin HRP which produces a soluble colored product after
• addition of TMB substrate.
• The enzyme reaction is stopped by dispensing of stop solution into the wells.The
• optical density （OD） of the solution at 450 nm is inversely proportional to the amount of bound Nilotinib
• molecule present in standards or samples.