83-2271-44　［受注停止］KRIBIOLISA Anti-EPO （Erythropoetin） ELISA 1 x 96 wells　KBI6200

特徴

• Description：ELISA for Accurate Quantitation of Anti-EPO from Human Serum and
• Plasma
• Erythropoietin （EPO） is a heavily glycosylated protein with a molecular weight of about 30,000 - 34,000 Daltons.
• Human EPO is a polypeptide consisting of 166 amino acids, containing one O-linked and three N-linked carbohydrate
• chains.
• The recombinant EPO is a good substitute for the native protein for use in an immunoassay.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The method employs enzyme immunoassay technique.
• Samples and standards are pipetted into microwell pre-coated
• with EPO antigen.
• After incubation and washing, HRP Conjugate is added to the wells.
• Anti-EPO present in the sample
• and standards will form a complex in the wells.
• Washing is done to remove the unbound HRP Conjugate.
• The ready to
• use substrate solution （TMB） is added to microwells and color develops directly proportional to the amount of Anti-
• EPO present in the standards and sample.
• Color development is then stopped by addition of stop solution.
• Absorbance is measured at 450 nm.