特徴

• Description：Immunoassay for quantitative estimation of VIP H-B-P-R in
• human serum, plasma and tissue extracts.
• VIP （Vasoactive intestinal peptide） is a peptide hormone that is vasoactive in the intestine.
• VIP is a peptide of
• 28 amino acid residues that belongs to a glucagon/secretin superfamily, the ligand of class II G protein–
• coupled receptors.
• VIP is produced in many tissues of vertebrates including the gut, pancreas, and
• suprachiasmatic nuclei of the hypothalamus in the brain.
• VIP stimulates contractility in the heart, causes
• vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of
• trachea, stomach and gall bladder.
• In humans, the vasoactive intestinal peptide is encoded by the VIP gene.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The VIP-H-B-P-R ELISA is a competitive immunoassay for the determination of VIP-H-B-P-R.
• The antiserum
• is captured by antibodies coated on a 96-well plate.
• A constant concentration of Biotin Concentrate and
• varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum.
• Captured Bt-tracer is subsequently bound by SA-HRP （streptavidin-conjugated horseradish peroxidase）,
• which produces a soluble colored product after a substrate is added.
• The enzyme reaction is stopped by
• dispensing an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution
• from blue to yellow.
• The optical density （OD） of the solution at 450 nm is inversely proportional to the amount
• of specific VIP-H-B-P-R bound.