83-2271-40　［受注停止］KRIBIOLISA Somatostatin （Stilamin） ELISA 1 x 96 wells　KBI5031

特徴

• Description：Immunoassay for quantitative estimation of Somatostatin in
• human serum, plasma and tissue extracts.
• Somatostatin, also known as growth hormone-inhibiting hormone （GHIH） or by several other names, is a
• peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via
• interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary
• hormones.
• Somatostatin inhibits insulin and glucagon secretion.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Somatostatin ELISA is a competitive immunoassay for the determination of Somatostatin.
• The antiserum
• is captured by antibodies coated on a 96-well plate.
• A constant concentration of Biotin Concentrate and
• varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum.
• Captured Bt-tracer is subsequently bound by SA-HRP （streptavidin-conjugated horseradish peroxidase）,
• which produces a soluble colored product after a substrate is added.
• The enzyme reaction is stopped by
• dispensing an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution
• from blue to yellow.
• The optical density （OD） of the solution at 450 nm is inversely proportional to the amount
• of specific Somatostatin bound.