特徴

• Description：Immunoassay for quantitative estimation of Human Secretin
• in human serum, plasma and tissue extracts.
• Secretin is a hormone that regulates water homeostasis throughout the body and influences the environment
• of the duodenum by regulating secretions in the stomach, pancreas, and liver.
• It is a peptide hormone
• produced in the S cells of the duodenum, which are located in the intestinal glands.
• In humans, the secretin
• peptide is encoded by the SCT gene.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Human Secretin ELISA is a competitive immunoassay for the determination of Human Secretin.
• The
• antiserum is captured by antibodies coated on a 96-well plate.
• A constant concentration of Biotin Concentrate
• and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum.
• Captured Bt-tracer is subsequently bound by SA-HRP （streptavidin-conjugated horseradish peroxidase）,
• which produces a soluble colored product after a substrate is added.
• The enzyme reaction is stopped by
• dispensing an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution
• from blue to yellow.
• The optical density （OD） of the solution at 450 nm is inversely proportional to the amount
• of specific Human Secretin bound.