83-2271-36　［受注停止］KRIBIOLISA Oxyntomodulin （Glucagon 37） ELISA 1 x 96 wells　KBI5026

特徴

• Description：Immunoassay for quantitative estimation of Oxyntomodulin
• in human serum, plasma and tissue extracts.
• Oxyntomodulin （Glucagon37） is a 37-amino acid polypeptide, consisting of the glucagon molecule plus a C-
• terminal extension.
• It is produced by ileal L cells after cleavage of proglucagon by prohormone convertase 1;
• mice lacking prohormone convertase 1 have a reduction in oxyntomodulin, GLP-1, GLP-2, and glicentin
• levels.
• As with GLP-1, oxyntomodulin is inactivated by DPP-4.
• Endogenous oxyntomodulin appears to work
• as a satiety signal, rising after a meal
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Oxyntomodulin ELISA is a competitive immunoassay for the determination of Oxyntomodulin.
• The
• antiserum is captured by antibodies coated on a 96-well plate.
• A constant concentration of Biotin Concentrate
• and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum.
• Captured Bt-tracer is subsequently bound by SA-HRP （streptavidin-conjugated horseradish peroxidase）,
• which produces a soluble colored product after a substrate is added.
• The enzyme reaction is stopped by
• dispensing an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution
• from blue to yellow.
• The optical density （OD） of the solution at 450 nm is inversely proportional to the amount
• of specific Oxyntomodulin bound.