83-2271-35　［受注停止］KRIBIOLISA Orexin A ELISA 1 x 96 wells　KBI5025

特徴

• Description：Immunoassay for quantitative estimation of Orexin-A in
• human serum, plasma and tissue extracts.
• Orexin-A, also known as hypocretin-1, is a naturally occurring neuropeptide and orexin isoform.
• The
• orexinergic nucleus in the lateral hypothalamus is the primary orexin projection system in the brain.
• Orexin-A
• is a peptide composed of 33 amino acids including an N-terminal pyroglutamyl residue and two intramolecular
• disulfide bridges between cysteine residues in 6 and 12 and 7 and 14 positions.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Orexin-A ELISA is a competitive immunoassay for the determination of Orexin-A.
• The antiserum is
• captured by antibodies coated on a 96-well plate.
• A constant concentration of Biotin Concentrate and varying
• concentrations of unlabeled standard or sample compete for binding specifically to the antiserum.
• Captured
• Bt-tracer is subsequently bound by SA-HRP （streptavidin-conjugated horseradish peroxidase）, which
• produces a soluble colored product after a substrate is added.
• The enzyme reaction is stopped by dispensing
• an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution from blue to
• yellow.
• The optical density （OD） of the solution at 450 nm is inversely proportional to the amount of specific
• Orexin-A bound.