Description：Immunoassay for quantitative estimation of Nafarelin in human serum, plasma and tissue extracts.
Nafarelin （Synarel）, is a gonadotropin-releasing hormone agonist （GnRH agonist）.
Its proposed mechanism of action is the desensitization of pituitary GnRH receptors leading to a decrease in gonadotropin release, and ovarian hormone serum concentrations similar to those achieved in postmenopausal women.
It decreases pituitary secretion of the gonadotropins luteinizing hormone （LH） and follicle stimulating hormone （FSH）.Nafarelin is also used in the treatment of estrogen-dependent conditions （such as endometriosis or uterine fibroids）, to treat central precocious puberty, or to control ovarian stimulation in in vitro fertilisation （IVF）.
About the kit：
- Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
- Recovery rates are between 85 - 115％
- Ready to use with a standard protocol with break-apart pre-coated wells
- Validated as per US FDA guidelines for Bioassays
- Optimized for matrix effects to ensure higher sensitivity.
- Shelf life： 1 year
The Nafarelin ELISA is a competitive immunoassay for the determination of Nafarelin.
The antiserum is captured by antibodies coated on a 96-well plate.
A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum.
Captured Bt-tracer is subsequently bound by SA-HRP （streptavidin-conjugated horseradish peroxidase）, which produces a soluble colored product after a substrate is added.
The enzyme reaction is stopped by dispensing an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution from blue to yellow.
The optical density （OD） of the solution at 450 nm is inversely proportional to the amount of specific Nafarelin bound.
