83-2271-29　［受注停止］KRIBIOLISA Leuprolide / Leuprorelin （Lupron） ELISA 1 x 96 wells　KBI5019

特徴

• Description：Immunoassay for quantitative estimation of Leuprolide in human serum.
• Leuprolide belongs to the general class of drugs known as hormones or hormone antagonists.
• It is a synthetic 9 residue peptide analog of gonadotropin releasing hormone.
• Leuprolide is used to treat advanced prostate cancer.
• It is also used to treat uterine fibroids and endometriosis.
• Leuprolide is also under investigation for possible use in the treatment of mild to moderate Alzheimer's disease.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Leuprolide ELISA is a competitive immunoassay for the determination of Leuprolide.
• The antiserum is captured by antibodies coated on a 96-well plate.
• A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum.
• Captured Bt-tracer is subsequently bound by SA-HRP （streptavidin-conjugated horseradish peroxidase）, which produces a soluble colored product after a substrate is added.
• The enzyme reaction is stopped by dispensing an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution from blue to yellow.
• The optical density （OD） of the solution at 450 nm is inversely proportional to the amount of specific Leuprolide bound.