83-2271-14　［受注停止］KRIBIOLISA Calcitonin （Salmon） （Miacalcic） ELISA 1 x 96 wells　KBI5004

特徴

• Description：Immunoassay for quantitative estimation of Calcitonin in human serum and plasma.
• Calcitonin （also known as thyrocalcitonin; Salmon/Miacalcic） is a 32-amino acid linear polypeptide hormone that is produced in humans primarily by the parafollicular cells （also known as C-cells） of the thyroid gland, and in many other animals in the ultimopharyngeal body.
• It acts to reduce blood calcium （Ca2+）, opposing the
• effects of parathyroid hormone （PTH）.
• Calcitonin injection is used to treat Paget's disease of bone, or high levels of calcium in the blood （hypercalcemia）.
• Calcitonin injection is also used to treat osteoporosis in postmenopausal women.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The Calcitonin ELISA is a sandwich immunoassay for the determination of BNP32.
• The antibodies coated on a 96-well plate capture the standard or sample.
• Calcitonin antiserum is subsequently bound to this complex.Biotin is then added followed by SA-HRP （streptavidin-conjugated horseradish peroxidase）, which produces a soluble colored product after a substrate is added.
• The enzyme reaction is stopped by dispensing an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution from blue to yellow.
• The optical density （OD） of the solution at 450 nm is directly proportional to the amount of specific Calcitonin bound.