83-2271-12　［受注停止］KRIBIOLISA BNP 32 （Human） （Natrecor） ELISA 1 x 96 wells　KBI5002

特徴

• Description：Immunoassay for quantitative estimation of BNP32 in human serum, plasma and tissue extracts.
• BNP32 （Nesiritide/Natrecor） is an endogenous peptide secreted from cardiac ventricles in response to volume increase and pressure overload that acts as an agonist at atrial natriuretic peptide （ANP） receptor A （NRP1）.In vitro： BNP 1-32 （10（-6） mol/L） activats cGMP.
• BNP significantly （P＜0.01） inhibited de novo collagen synthesis as assessed by [3H]proline incorporation, whereas zymographic MMP-2 （gelatinase） abundance was significantly （P＜0.05） stimulated by BNP between 10（-7） and 10（-6） mol/L.
• Decreases de novo collagen synthesis and increases MMP gene expression in vitro.
• In vivo： inhibits the sympathetic and renin-angiotensin-aldosterone systems.
• About the kit：
• - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
• - Recovery rates are between 85 - 115％
• - Ready to use with a standard protocol with break-apart pre-coated wells
• - Validated as per US FDA guidelines for Bioassays
• - Optimized for matrix effects to ensure higher sensitivity.
• - Shelf life： 1 year
• The BNP32 ELISA is a sandwich immunoassay for the determination of BNP32.
• The antibodies coated on a 96-well plate capture the standard or sample.
• BNP32 antiserum is subsequently bound to this complex.
• Biotin is then added followed by SA-HRP （streptavidin-conjugated horseradish peroxidase）, which produces a soluble colored product after a substrate is added.
• The enzyme reaction is stopped by dispensing an acidic solution （H2SO4） into the wells after 10 min at room temperature turning the solution from blue to yellow.
• The optical density （OD） of the solution at 450 nm is directly proportional to the amount of specific BNP32 bound.