Description：Enzyme Immunoassay for the quantitative determination of Ranibizumab in serum and plasma.
This kit measures the total drug （free, bound or partially bound）
Ranibizumab （Lucentis） is a recombinant humanized IgG1 monoclonal antibody fragment that binds to and inhibits vascular endothelial growth factor A （VEGF-A）.
VEGF is a biochemical signal protein that promotes angiogenesis throughout the body and in the eye.
Through binding to VEGF-A, ranibizumab interrupts the interaction of VEGF with its receptors, and thus prevents the subsequent growth of new blood vessels.
Krishgen PK kits, developed using the innovator drug as calibrator, are used for the quantitation of serum protein drug levels, and can be used for biosimilar and generics research as well.
About the kit：
- Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
- Recovery rates are between 85 - 115％
- Ready to use with a standard protocol with break-apart pre-coated wells
- Validated as per US FDA guidelines for Bioassays
- Optimized for matrix effects to ensure higher sensitivity.
- Shelf life： 1 year
The method employs the quantitative sandwich enzyme immunoassay technique.
Antibodies to Ranibizumab are pre-coated onto microwells.
HRP Conjugate, Samples / Standards are pipetted into microwells and human Ranibizumab present in the sample are bound by the capture antibody.
After washing microwells in order to remove any non-specific binding, the ready to use substrate solution （TMB） is added to microwells and color develops proportionally to the amount of Ranibizumab in the sample.
Color development is then stopped by addition of stop solution.
