Total assay solution for plate-based, high-throughput measurements of intracellular sodium flux.
Also endpoint assay compatible.Excitation： 525 nm | Emission： 545 nmION's Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na⁺ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters.
In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na⁺.
ION's Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells.
The optional use of a probenecid solution and an extracellular background masking solution （TRS） offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na⁺ indicators （e.g. Chinese Hamster Ovary, CHO cells） and when performing assays in complete, serum-containing cell culture medium is desired.ION's Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein （YFP）.
